Journal: Stem Cell Research & Therapy

Article Title: Compared to the amniotic membrane, Wharton’s jelly may be a more suitable source of mesenchymal stem cells for cardiovascular tissue engineering and clinical regeneration

doi: 10.1186/s13287-017-0501-x

Figure Lengend Snippet: Representative images of multi-lineage differentiation potential of isolated WJ-MSCs and AM-MSCs. WJ-MSCs and AM-MSCs were induced to differentiate toward adipogenic lineage and verified by Alizarin Red S staining (magnification, ×100), osteogenic lineage and verified by Oil Red O staining (magnification, ×200), and chondrogenic lineage and verified by Alcian Blue staining (magnification, ×200). Scale bar, 100 μm. AM-MSCs amniotic membrane mesenchymal stem cells, WJ-MSCs Wharton’s jelly mesenchymal stem cells

Article Snippet: Sections (4 μm) were deparaffinized in xylene and stained with Alcian Blue Staining Kit (ScienCell, Carlsbad, CA, USA) according to the user’s manual.

Techniques: Isolation, Staining, Membrane

Journal: The Journal of Clinical Investigation

Article Title: Reprogramming dysfunctional CD8 + T cells to promote properties associated with natural HIV control

doi: 10.1172/JCI157549

Figure Lengend Snippet: Total CD8 + T cells from individuals without HIV were treated with medium, vehicle (Veh.) control, or the GSK3 inhibitor (inh), followed by incubation under basal conditions or with anti-CD3/anti-CD28 stimulation for 48 hours. ( A and B ) Analysis of CD8 + T cell subpopulations in unstimulated cells ( n = 4). ( C ) Fold change of CD8 + T cell subpopulations upon vehicle control or GSK3 inhibitor treatment, relative to the medium alone condition ( n = 4). ( D ) Expression of TCF-1 in CD8 + T cell subsets ( n = 4). ( E ) Fold change in the expression of the indicated markers induced by anti-CD3/anti-CD28 antibody stimulation relative to unstimulated cells ( n = 5). ( F ) Analysis of dead cells by Aqua LIVE/DEAD + staining (Aqua L/D) among total and T-bet + CD8 + T cells, and fold change in dead CD8 + T cells induced by anti-CD3/anti-CD28 stimulation relative to the unstimulated (Unstim.) condition ( n = 5). ( G ) Frequencies of granzyme B + (GZMB + ), IL-2 + , IFN-γ + , and TNF-α + CD8 + T cells after anti-CD3/anti-CD28 stimulation. ( H ) Expression of 1 to 4 functions in CD8 + T cells ( n = 5). * P < 0.05, by Dunn’s test ( B ) and Wilcoxon test ( C , D , and F – H ). Data obtained from 2 ( A – F ) or 3 ( G and H ) independent experiments are shown.

Article Snippet: Purified CD8 + T cells were stained with the LIVE/DEAD Fixable Aqua Dead Cell Stain kit and the following antibodies: anti–CD3 Alexa Fluor 700, anti–CD8 APC Cy7, anti–CCR7 PE Cy7, anti–CD45RA BV421, and anti–CD27 PE (all from BD Biosciences).

Techniques: Incubation, Expressing, Staining